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Site-specific mutagenesis using asymmetric polymerase chain reaction and a single mutant primer.

A method is described for preparing site-specific mutants using a polymerase chain reaction (PCR) based protocol. The protocol requires a single mutant primer, and has been used to introduce mutations into DNA fragments ranging in size from 200 bp to 1569 bp in length in the GM-CSF, beta-actin, huma...

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Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Cyhoeddwyd yn:Nucleic Acids Res
Prif Awduron: Perrin, S, Gilliland, G
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: Oxford University Press 1990
Mynediad Ar-lein:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC332882/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2259632/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/18.24.7433
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