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Site-specific mutagenesis using asymmetric polymerase chain reaction and a single mutant primer.
A method is described for preparing site-specific mutants using a polymerase chain reaction (PCR) based protocol. The protocol requires a single mutant primer, and has been used to introduce mutations into DNA fragments ranging in size from 200 bp to 1569 bp in length in the GM-CSF, beta-actin, huma...
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| Main Authors: | , |
|---|---|
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
1990
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| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC332882/ https://ncbi.nlm.nih.gov/pubmed/2259632 |
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