Site-specific mutagenesis using asymmetric polymerase chain reaction and a single mutant primer.
A method is described for preparing site-specific mutants using a polymerase chain reaction (PCR) based protocol. The protocol requires a single mutant primer, and has been used to introduce mutations into DNA fragments ranging in size from 200 bp to 1569 bp in length in the GM-CSF, beta-actin, huma...
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| Vydáno v: | Nucleic Acids Res |
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| Hlavní autoři: | , |
| Médium: | Artigo |
| Jazyk: | Inglês |
| Vydáno: |
Oxford University Press
1990
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| On-line přístup: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC332882/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2259632/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/18.24.7433 |
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