Fluorescent Recovery after Photobleaching (FRAP) Analysis of Nuclear Export Rates Identifies Intrinsic Features of Nucleocytoplasmic Transport

A quantitative description of carrier-mediated nuclear export in live cells is presented. To this end, we fused a prototypical leucine-rich nuclear export signal (NES) to GFP as a cargo model and expressed the fluorescent chimera in live CHO-K1 cells. By modeling FRAP data, we calculate the NES affi...

Deskribapen osoa

Gorde:
Xehetasun bibliografikoak
Egile Nagusiak: Cardarelli, Francesco, Tosti, Luca, Serresi, Michela, Beltram, Fabio, Bizzarri, Ranieri
Formatua: Artigo
Hizkuntza:Inglês
Argitaratua: American Society for Biochemistry and Molecular Biology 2012
Gaiak:
Molecular Biophysics
Sarrera elektronikoa:https://ncbi.nlm.nih.gov/pmc/articles/PMC3325589/
https://ncbi.nlm.nih.gov/pubmed/22190681
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.M111.304899
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