Probing Nuclear Localization Signal-Importin α Binding Equilibria in Living Cells

The regulated process of protein import into the nucleus of a eukaryotic cell is mediated by specific nuclear localization signals (NLSs) that are recognized by protein-import receptors. In this study, we present fluorescence-based methods to quantitatively address the physicochemical details of NLS...

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Библиографические подробности
Главные авторы: Cardarelli, Francesco, Bizzarri, Ranieri, Serresi, Michela, Albertazzi, Lorenzo, Beltram, Fabio
Формат: Artigo
Язык:Inglês
Опубликовано: American Society for Biochemistry and Molecular Biology 2009
Предметы:
Biomolecular Networks
Online-ссылка:https://ncbi.nlm.nih.gov/pmc/articles/PMC2794778/
https://ncbi.nlm.nih.gov/pubmed/19858191
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.M109.036699
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