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Probing Nuclear Localization Signal-Importin α Binding Equilibria in Living Cells
The regulated process of protein import into the nucleus of a eukaryotic cell is mediated by specific nuclear localization signals (NLSs) that are recognized by protein-import receptors. In this study, we present fluorescence-based methods to quantitatively address the physicochemical details of NLS...
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| Hlavní autoři: | , , , , |
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| Médium: | Artigo |
| Jazyk: | Inglês |
| Vydáno: |
American Society for Biochemistry and Molecular Biology
2009
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| Témata: | |
| On-line přístup: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2794778/ https://ncbi.nlm.nih.gov/pubmed/19858191 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.M109.036699 |
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