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Differential fluorescence labeling of cysteinyl clusters uncovers high tissue levels of thionein
The isolation of thionein (T) from tissues has not been reported heretofore. T contains 20 cysteinyl residues that react with 7-fluorobenz-2-oxa-1,3-diazole-4-sulfonamide to form fluorescent adducts. In metallothionein (MT) the cysteinyl residues, which are bound to zinc, do not react. However, they...
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| Autors principals: | , , |
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| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
The National Academy of Sciences
2001
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC33251/ https://ncbi.nlm.nih.gov/pubmed/11331777 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.101123298 |
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