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Differential fluorescence labeling of cysteinyl clusters uncovers high tissue levels of thionein

The isolation of thionein (T) from tissues has not been reported heretofore. T contains 20 cysteinyl residues that react with 7-fluorobenz-2-oxa-1,3-diazole-4-sulfonamide to form fluorescent adducts. In metallothionein (MT) the cysteinyl residues, which are bound to zinc, do not react. However, they...

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Detalhes bibliográficos
Main Authors: Yang, Yi, Maret, Wolfgang, Vallee, Bert L.
Formato: Artigo
Idioma:Inglês
Publicado em: The National Academy of Sciences 2001
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC33251/
https://ncbi.nlm.nih.gov/pubmed/11331777
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.101123298
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