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Protein quantification from complex protein mixtures using a proteomics methodology with single-cell resolution

We have developed an extremely sensitive technique, termed immuno-detection amplified by T7 RNA polymerase (IDAT) that is capable of monitoring proteins, lipids, and metabolites and their modifications at the single-cell level. A double-stranded oligonucleotide containing the T7 promoter is conjugat...

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Detalhes bibliográficos
Main Authors: Zhang, Hong-Tao, Kacharmina, Janet E., Miyashiro, Kevin, Greene, Mark I., Eberwine, James
Formato: Artigo
Idioma:Inglês
Publicado em: The National Academy of Sciences 2001
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC33241/
https://ncbi.nlm.nih.gov/pubmed/11320219
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.101124598
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