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Protein quantification from complex protein mixtures using a proteomics methodology with single-cell resolution
We have developed an extremely sensitive technique, termed immuno-detection amplified by T7 RNA polymerase (IDAT) that is capable of monitoring proteins, lipids, and metabolites and their modifications at the single-cell level. A double-stranded oligonucleotide containing the T7 promoter is conjugat...
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Main Authors: | , , , , |
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Formato: | Artigo |
Idioma: | Inglês |
Publicado em: |
The National Academy of Sciences
2001
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Assuntos: | |
Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC33241/ https://ncbi.nlm.nih.gov/pubmed/11320219 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.101124598 |
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