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Relative quantification of peptide phosphorylation in a complex mixture using (18)O labeling
We have developed a method to determine the degree of phosphorylation of a peptide in a complex mixture without enrichment or operation of the mass spectrometer in negative ion mode. Yeast lysate containing known amounts of synthetic peptides (VPQLEIVPNSAEERLHSMK and VPQLEIVPN[pS]AEERLHSMK) was labe...
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| Huvudupphovsmän: | , , |
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| Materialtyp: | Artigo |
| Språk: | Inglês |
| Publicerad: |
2007
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| Ämnen: | |
| Länkar: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2771636/ https://ncbi.nlm.nih.gov/pubmed/17684036 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1152/physiolgenomics.00096.2007 |
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