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Improvement of PCR reaction conditions for site-directed mutagenesis of big plasmids
QuickChange mutagenesis is the method of choice for site-directed mutagenesis (SDM) of target sequences in a plasmid. It can be applied successfully to small plasmids (up to 10 kb). However, this method cannot efficiently mutate bigger plasmids. Using KOD Hot Start polymerase in combination with hig...
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| 主要な著者: | , , |
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| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
Zhejiang University Press
2012
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| 主題: | |
| オンライン･アクセス: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3323938/ https://ncbi.nlm.nih.gov/pubmed/22467364 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1631/jzus.B1100180 |
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