Wordt geladen...
Improvement of PCR reaction conditions for site-directed mutagenesis of big plasmids
QuickChange mutagenesis is the method of choice for site-directed mutagenesis (SDM) of target sequences in a plasmid. It can be applied successfully to small plasmids (up to 10 kb). However, this method cannot efficiently mutate bigger plasmids. Using KOD Hot Start polymerase in combination with hig...
Bewaard in:
| Hoofdauteurs: | , , |
|---|---|
| Formaat: | Artigo |
| Taal: | Inglês |
| Gepubliceerd in: |
Zhejiang University Press
2012
|
| Onderwerpen: | |
| Online toegang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3323938/ https://ncbi.nlm.nih.gov/pubmed/22467364 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1631/jzus.B1100180 |
| Tags: |
Voeg label toe
Geen labels, Wees de eerste die dit record labelt!
|