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Disruption of phase during PCR amplification and cloning of heterozygous target sequences.
PCR amplification of genomic DNA or cDNA has become a standard tool for identification of mutations underlying genetic disease. There are inherent limitations in the application of this method in compound heterozygotes. One problem which is encountered is the disruption of phase (linkage) between he...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
1990
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| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC332136/ https://ncbi.nlm.nih.gov/pubmed/1976239 |
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