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Termination of DNA synthesis by novel 3'-modified-deoxyribonucleoside 5'-triphosphates.

Eight 3'-modified-dNTPs were synthesized and tested in two different DNA template assays for incorporation activity. From this enzymatic screen, two 3'-O-methyl-dNTPs were shown to terminate DNA syntheses mediated by a number of polymerases and may be used as alternative terminators in Sanger sequen...

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Udgivet i:Nucleic Acids Res
Principais autores: Metzker, M L, Raghavachari, R, Richards, S, Jacutin, S E, Civitello, A, Burgess, K, Gibbs, R A
Format: Artigo
Sprog:Inglês
Udgivet: Oxford University Press 1994
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC331938/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7937154/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/22.20.4259
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