Termination of DNA synthesis by novel 3'-modified-deoxyribonucleoside 5'-triphosphates.
Eight 3'-modified-dNTPs were synthesized and tested in two different DNA template assays for incorporation activity. From this enzymatic screen, two 3'-O-methyl-dNTPs were shown to terminate DNA syntheses mediated by a number of polymerases and may be used as alternative terminators in Sanger sequen...
Kaydedildi:
| Yayımlandı: | Nucleic Acids Res |
|---|---|
| Asıl Yazarlar: | , , , , , , |
| Materyal Türü: | Artigo |
| Dil: | Inglês |
| Baskı/Yayın Bilgisi: |
Oxford University Press
1994
|
| Online Erişim: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC331938/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7937154/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/22.20.4259 |
| Etiketler: |
Etiket eklenmemiş, İlk siz ekleyin!
|