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Highly Perturbed pK(a) Values in the Unfolded State of Hen Egg White Lysozyme

The majority of pK(a) values in protein unfolded states are close to the amino acid model pK(a) values, thus reflecting the weak intramolecular interactions present in the unfolded ensemble of most proteins. We have carried out thermal denaturation measurements on the WT and eight mutants of HEWL fr...

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Detalhes bibliográficos
Main Authors: Bradley, John, O'Meara, Fergal, Farrell, Damien, Nielsen, Jens Erik
Formato: Artigo
Idioma:Inglês
Publicado em: The Biophysical Society 2012
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3318136/
https://ncbi.nlm.nih.gov/pubmed/22500764
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bpj.2012.02.048
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