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Structure-guided evolution of cyan fluorescent proteins towards a quantum yield of 93%

Cyan variants of green fluorescent protein are widely used as donors in Förster resonance energy transfer experiments. The popular, but modestly bright, Enhanced Cyan Fluorescent Protein (ECFP) was sequentially improved into the brighter variants Super Cyan Fluorescent Protein 3A (SCFP3A) and mTurqu...

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Detalhes bibliográficos
Main Authors: Goedhart, Joachim, von Stetten, David, Noirclerc-Savoye, Marjolaine, Lelimousin, Mickaël, Joosen, Linda, Hink, Mark A., van Weeren, Laura, Gadella, Theodorus W.J., Royant, Antoine
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Pub. Group 2012
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3316892/
https://ncbi.nlm.nih.gov/pubmed/22434194
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/ncomms1738
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