Quantitative Co-Expression of Proteins at the Single Cell Level – Application to a Multimeric FRET Sensor

BACKGROUND: Co-expression of proteins is generally achieved by introducing two (or more) independent plasmids into cells, each driving the expression of a different protein of interest. However, the relative expression levels may vary strongly between individual cells and cannot be controlled. Ideal...

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Detalhes bibliográficos
Main Authors: Goedhart, Joachim, van Weeren, Laura, Adjobo-Hermans, Merel J.W., Elzenaar, Ies, Hink, Mark A., Gadella, Theodorus W.J.
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2011
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3219669/
https://ncbi.nlm.nih.gov/pubmed/22114669
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0027321
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