A FRET-based biosensor for measuring Gα13 activation in single cells

Förster Resonance Energy Transfer (FRET) provides a way to directly observe the activation of heterotrimeric G-proteins by G-protein coupled receptors (GPCRs). To this end, FRET based biosensors are made, employing heterotrimeric G-protein subunits tagged with fluorescent proteins. These FRET based...

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Detalles Bibliográficos
Publicado en:PLoS One
Main Authors: Mastop, Marieke, Reinhard, Nathalie R., Zuconelli, Cristiane R., Terwey, Fenna, Gadella, Theodorus W. J., van Unen, Jakobus, Adjobo-Hermans, Merel J. W., Goedhart, Joachim
Formato: Artigo
Idioma:Inglês
Publicado: Public Library of Science 2018
Assuntos:
Research Article
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC5837189/
https://ncbi.nlm.nih.gov/pubmed/29505611
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0193705
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