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Construction of mutant and chimeric genes using the polymerase chain reaction.
In the polymerase chain reaction (PCR) the specific amplification of a small segment of DNA within a complex DNA sample is effected by repeated cycles of DNA denaturation and enzymatic synthesis primed by two oligonucleotides complementary to regions within opposite strands of the DNA. In this repor...
Uloženo v:
| Hlavní autoři: | , , , |
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| Médium: | Artigo |
| Jazyk: | Inglês |
| Vydáno: |
1989
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| On-line přístup: | https://ncbi.nlm.nih.gov/pmc/articles/PMC331615/ https://ncbi.nlm.nih.gov/pubmed/2915928 |
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