Quantitation of mRNA by the polymerase chain reaction.
A method for the quantitation of specific mRNA species by the polymerase chain reaction (PCR) has been developed by using a synthetic RNA as an internal standard. The specific target mRNA and the internal standard are coamplified in one reaction in which the same primers are used. The amount of mRNA...
Enregistré dans:
| Publié dans: | Proc Natl Acad Sci U S A |
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| Auteurs principaux: | , , |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
National Academy of Sciences
1989
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC298572/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2481313/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.86.24.9717 |
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