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Quantitation of mRNA by the polymerase chain reaction.

A method for the quantitation of specific mRNA species by the polymerase chain reaction (PCR) has been developed by using a synthetic RNA as an internal standard. The specific target mRNA and the internal standard are coamplified in one reaction in which the same primers are used. The amount of mRNA...

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Udgivet i:Proc Natl Acad Sci U S A
Principais autores: Wang, A M, Doyle, M V, Mark, D F
Format: Artigo
Sprog:Inglês
Udgivet: National Academy of Sciences 1989
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC298572/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2481313/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.86.24.9717
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