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Quantitation of mRNA by the polymerase chain reaction.
A method for the quantitation of specific mRNA species by the polymerase chain reaction (PCR) has been developed by using a synthetic RNA as an internal standard. The specific target mRNA and the internal standard are coamplified in one reaction in which the same primers are used. The amount of mRNA...
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Main Authors: | , , |
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Format: | Artigo |
Language: | Inglês |
Published: |
1989
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Subjects: | |
Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC298572/ https://ncbi.nlm.nih.gov/pubmed/2481313 |
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