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Quantitation of mRNA by the polymerase chain reaction.

A method for the quantitation of specific mRNA species by the polymerase chain reaction (PCR) has been developed by using a synthetic RNA as an internal standard. The specific target mRNA and the internal standard are coamplified in one reaction in which the same primers are used. The amount of mRNA...

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Bibliographic Details
Main Authors: Wang, A M, Doyle, M V, Mark, D F
Format: Artigo
Language:Inglês
Published: 1989
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC298572/
https://ncbi.nlm.nih.gov/pubmed/2481313
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