A Quantitative, High-Throughput Reverse Genetic Screen Reveals Novel Connections between Pre–mRNA Splicing and 5′ and 3′ End Transcript Determinants

Here we present the development and implementation of a genome-wide reverse genetic screen in the budding yeast, Saccharomyces cerevisiae, that couples high-throughput strain growth, robotic RNA isolation and cDNA synthesis, and quantitative PCR to allow for a robust determination of the level of ne...

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Detalhes bibliográficos
Main Authors: Albulescu, Laura-Oana, Sabet, Nevin, Gudipati, Mohanram, Stepankiw, Nicholas, Bergman, Zane J., Huffaker, Tim C., Pleiss, Jeffrey A.
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2012
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3315463/
https://ncbi.nlm.nih.gov/pubmed/22479188
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pgen.1002530
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