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Multiplexed Primer Extension sequencing: a targeted RNA-seq method that enables high-precision quantitation of mRNA splicing isoforms and rare pre-mRNA splicing intermediates
The study of pre-mRNA splicing has been greatly aided by the advent of RNA sequencing (RNA-seq), which enables the genome-wide detection of discrete splice isoforms. Quantification of these splice isoforms requires analysis of splicing informative sequencing reads, those that unambiguously map to a...
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| Publicado en: | Methods |
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| Autores principales: | , , |
| Formato: | Artigo |
| Lenguaje: | Inglês |
| Publicado: |
2019
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| Materias: | |
| Acceso en línea: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6868320/ https://ncbi.nlm.nih.gov/pubmed/31121301 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ymeth.2019.05.013 |
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