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Detection of splice isoforms and rare intermediates using multiplexed primer extension sequencing
Targeted RNA-sequencing aims to focus coverage on areas of interest that are inadequately sampled in standard RNA-sequencing experiments. Here we present a novel approach for targeted RNA-sequencing that uses complex pools of reverse transcription primers to enable sequencing enrichment at user-sele...
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| Publié dans: | Nat Methods |
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| Auteurs principaux: | , , , , |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
2018
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6414223/ https://ncbi.nlm.nih.gov/pubmed/30573814 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41592-018-0258-x |
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