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Binding of nucleic acids to E. coli RNase HI observed by NMR and CD spectroscopy.

To clarify the mechanism by which the RNA portion of a DNA/RNA hybrid is specifically hydrolyzed by ribonuclease H (RNase H), the binding of a DNA/RNA hybrid, a DNA/DNA duplex, or an RNA/RNA duplex to RNase HI from Escherichia coli was investigated by 1H-15N heteronuclear NMR. Chemical shift changes...

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Udgivet i:Nucleic Acids Res
Principais autores: Oda, Y, Iwai, S, Ohtsuka, E, Ishikawa, M, Ikehara, M, Nakamura, H
Format: Artigo
Sprog:Inglês
Udgivet: Oxford University Press 1993
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC331492/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7694232/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/21.20.4690
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