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The double role of methyl donor and allosteric effector of S-adenosyl-methionine for Dam methylase of E. coli.
The turnover of DNA-adenine-methylase of E. coli strongly decreases when the temperature is lowered. This has allowed us to study the binding of Dam methylase on 14 bp DNA fragments at 0 degrees C by gel retardation in the presence of Ado-Met, but without methylation taking place. The enzyme can bin...
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| 主要な著者: | , |
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| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
1990
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| オンライン・アクセス: | https://ncbi.nlm.nih.gov/pmc/articles/PMC331253/ https://ncbi.nlm.nih.gov/pubmed/2201947 |
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