The double role of methyl donor and allosteric effector of S-adenosyl-methionine for Dam methylase of E. coli.
The turnover of DNA-adenine-methylase of E. coli strongly decreases when the temperature is lowered. This has allowed us to study the binding of Dam methylase on 14 bp DNA fragments at 0 degrees C by gel retardation in the presence of Ado-Met, but without methylation taking place. The enzyme can bin...
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| Publicado en: | Nucleic Acids Res |
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| Autores principales: | , |
| Formato: | Artigo |
| Lenguaje: | Inglês |
| Publicado: |
Oxford University Press
1990
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| Acceso en línea: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC331253/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2201947/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/18.15.4369 |
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