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The double role of methyl donor and allosteric effector of S-adenosyl-methionine for Dam methylase of E. coli.

The turnover of DNA-adenine-methylase of E. coli strongly decreases when the temperature is lowered. This has allowed us to study the binding of Dam methylase on 14 bp DNA fragments at 0 degrees C by gel retardation in the presence of Ado-Met, but without methylation taking place. The enzyme can bin...

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Библиографические подробности
Главные авторы: Bergerat, A, Guschlbauer, W
Формат: Artigo
Язык:Inglês
Опубликовано: 1990
Online-ссылка:https://ncbi.nlm.nih.gov/pmc/articles/PMC331253/
https://ncbi.nlm.nih.gov/pubmed/2201947
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