Efficient Large Volume Lentiviral Vector Production Using Flow Electroporation

Lentiviral vectors are beginning to emerge as a viable choice for human gene therapy. Here, we describe a method that combines the convenience of a suspension cell line with a scalable, nonchemically based, and GMP-compliant transfection technique known as flow electroporation (EP). Flow EP paramete...

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Detalhes bibliográficos
Main Authors: Witting, Scott R., Li, Lin-Hong, Jasti, Aparna, Allen, Cornell, Cornetta, Kenneth, Brady, James, Shivakumar, Rama, Peshwa, Madhusudan V.
Formato: Artigo
Idioma:Inglês
Publicado em: Mary Ann Liebert, Inc. 2012
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Brief Reports
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3311912/
https://ncbi.nlm.nih.gov/pubmed/21933028
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1089/hum.2011.088
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