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Efficient Large Volume Lentiviral Vector Production Using Flow Electroporation
Lentiviral vectors are beginning to emerge as a viable choice for human gene therapy. Here, we describe a method that combines the convenience of a suspension cell line with a scalable, nonchemically based, and GMP-compliant transfection technique known as flow electroporation (EP). Flow EP paramete...
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Main Authors: | , , , , , , , |
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Formato: | Artigo |
Idioma: | Inglês |
Publicado em: |
Mary Ann Liebert, Inc.
2012
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Assuntos: | |
Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3311912/ https://ncbi.nlm.nih.gov/pubmed/21933028 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1089/hum.2011.088 |
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