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Fast solid support detection of PCR amplified viral DNA sequences using radioiodinated or hapten labelled primers.

Oligonucleotides with novel modifications have been synthesized and incorporated into enzymatically amplified DNA sequences. They allow the fast detection of viral DNA sequences after two rounds of amplification. The hybrids formed are immobilized by affinity on coated tubes and detected by direct b...

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Udgivet i:Nucleic Acids Res
Principais autores: Sauvaigo, S, Fouqué, B, Roget, A, Livache, T, Bazin, H, Chypre, C, Téoule, R
Format: Artigo
Sprog:Inglês
Udgivet: Oxford University Press 1990
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC330921/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2162518/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/18.11.3175
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