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Reversible Major Histocompatibility Complex I-Peptide Multimers Containing Ni(2+)-Nitrilotriacetic Acid Peptides and Histidine Tags Improve Analysis and Sorting of CD8(+) T Cells
MHC-peptide multimers containing biotinylated MHC-peptide complexes bound to phycoerythrin (PE) streptavidin (SA) are widely used for analyzing and sorting antigen-specific T cells. Here we describe alternative T cell-staining reagents that are superior to conventional reagents. They are built on re...
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| Main Authors: | , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
American Society for Biochemistry and Molecular Biology
2011
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3308881/ https://ncbi.nlm.nih.gov/pubmed/21990358 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.M111.283127 |
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