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Transgenic expression and purification of myosin isoforms using the Drosophila melanogaster indirect flight muscle system

Biophysical and structural studies on muscle myosin rely upon milligram quantities of extremely pure material. However, many biologically interesting myosin isoforms are expressed at levels that are too low for direct purification from primary tissues. Efforts aimed at recombinant expression of func...

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Detalhes bibliográficos
Main Authors: Caldwell, James T., Melkani, Girish C., Huxford, Tom, Bernstein, Sanford I.
Formato: Artigo
Idioma:Inglês
Publicado em: 2011
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3308726/
https://ncbi.nlm.nih.gov/pubmed/22178692
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ymeth.2011.12.002
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