Detecting base pair substitutions in DNA fragments by temperature-gradient gel electrophoresis.

A vertical gel electrophoresis apparatus is described which can distinguish DNA fragments differing by single base pair substitutions. The system employs a homogenous polyacrylamide gel containing urea-formamide and a temperature gradient which runs either perpendicular or parallel to the direction...

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Bibliographic Details

Published in:	Nucleic Acids Res
Main Authors:	Wartell, R M, Hosseini, S H, Moran, C P
Format:	Artigo
Language:	Inglês
Published:	Oxford University Press 1990
Online Access:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC330754/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2339057/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/18.9.2699
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