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Perturbation of DNA hairpins containing the EcoRI recognition site by hairpin loops of varying size and composition: physical (NMR and UV) and enzymatic (EcoRI) studies.

We have investigated loop-induced structural perturbation of the stem structure in hairpins d(GAATTCXnGAATTC) (X = A, T and n = 3, 4, 5 and 6) that contain an EcoRI restriction site in close proximity to the hairpin loop. Oligonucleotides containing either a T3 or a A3 loop were not hydrolyzed by th...

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Autori principali: Germann, M W, Kalisch, B W, Lundberg, P, Vogel, H J, van de Sande, J H
Natura: Artigo
Lingua:Inglês
Pubblicazione: 1990
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC330516/
https://ncbi.nlm.nih.gov/pubmed/2326190
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