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Purification and characterization of the in vitro activity of I-Sce I, a novel and highly specific endonuclease encoded by a group I intron.
Group I intron encoded proteins represent a novel class of site specific double strand endonucleases. The endonuclease activity of this class of proteins has been first demonstrated in vivo for I-Sce I which is encoded by a mitochondrial intron of Saccharomyces cerevisiae. Assays using crude cell ex...
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| Published in: | Nucleic Acids Res |
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| Main Authors: | , , , , |
| Format: | Artigo |
| Language: | Inglês |
| Published: |
Oxford University Press
1990
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| Online Access: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC330504/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2183191/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/18.6.1407 |
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