Purification and characterization of the in vitro activity of I-Sce I, a novel and highly specific endonuclease encoded by a group I intron.

Group I intron encoded proteins represent a novel class of site specific double strand endonucleases. The endonuclease activity of this class of proteins has been first demonstrated in vivo for I-Sce I which is encoded by a mitochondrial intron of Saccharomyces cerevisiae. Assays using crude cell ex...

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Pubblicato in:	Nucleic Acids Res
Autori principali:	Monteilhet, C, Perrin, A, Thierry, A, Colleaux, L, Dujon, B
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	Oxford University Press 1990
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC330504/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2183191/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/18.6.1407
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