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Purification and characterization of the in vitro activity of I-Sce I, a novel and highly specific endonuclease encoded by a group I intron.

Group I intron encoded proteins represent a novel class of site specific double strand endonucleases. The endonuclease activity of this class of proteins has been first demonstrated in vivo for I-Sce I which is encoded by a mitochondrial intron of Saccharomyces cerevisiae. Assays using crude cell ex...

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Autori principali: Monteilhet, C, Perrin, A, Thierry, A, Colleaux, L, Dujon, B
Natura: Artigo
Lingua:Inglês
Pubblicazione: 1990
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC330504/
https://ncbi.nlm.nih.gov/pubmed/2183191
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