Measurement by quantitative PCR of changes in HPRT, PGK-1, PGK-2, APRT, MTase, and Zfy gene transcripts during mouse spermatogenesis.

A reverse transcriptase-polymerase chain reaction assay (RT-PCR) was used quantitatively to measure accumulated levels of RNA transcripts in total mouse RNAs derived from male germ cells at various spermatogenic stages. RNA levels for two X-linked enzymes, phosphoglycerate kinase (PGK-1) and hypoxan...

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Bibliografische Detailangaben

Veröffentlicht in:	Nucleic Acids Res
Hauptverfasser:	Singer-Sam, J, Robinson, M O, Bellvé, A R, Simon, M I, Riggs, A D
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	Oxford University Press 1990
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC330442/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1690874/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/18.5.1255
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