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Measurement by quantitative PCR of changes in HPRT, PGK-1, PGK-2, APRT, MTase, and Zfy gene transcripts during mouse spermatogenesis.

A reverse transcriptase-polymerase chain reaction assay (RT-PCR) was used quantitatively to measure accumulated levels of RNA transcripts in total mouse RNAs derived from male germ cells at various spermatogenic stages. RNA levels for two X-linked enzymes, phosphoglycerate kinase (PGK-1) and hypoxan...

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Detalhes bibliográficos
Main Authors: Singer-Sam, J, Robinson, M O, Bellvé, A R, Simon, M I, Riggs, A D
Formato: Artigo
Idioma:Inglês
Publicado em: 1990
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC330442/
https://ncbi.nlm.nih.gov/pubmed/1690874
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