Llwytho...
Hexamer to monomer equilibrium of E. coli Hfq in solution and its impact on RNA annealing
The bacterial Sm-like protein Hfq forms a ring shaped homo-hexamer that is necessary for Hfq to bind nucleic acids and to act in sRNA regulation. Using semi-native gels and fluorescence anisotropy, we show that Hfq undergoes a cooperative conformational change from monomer to hexamer around 1 μM pro...
Wedi'i Gadw mewn:
| Prif Awduron: | , |
|---|---|
| Fformat: | Artigo |
| Iaith: | Inglês |
| Cyhoeddwyd: |
2012
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| Pynciau: | |
| Mynediad Ar-lein: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3303956/ https://ncbi.nlm.nih.gov/pubmed/22326348 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jmb.2012.02.009 |
| Tagiau: |
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