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Hexamer to monomer equilibrium of E. coli Hfq in solution and its impact on RNA annealing
The bacterial Sm-like protein Hfq forms a ring shaped homo-hexamer that is necessary for Hfq to bind nucleic acids and to act in sRNA regulation. Using semi-native gels and fluorescence anisotropy, we show that Hfq undergoes a cooperative conformational change from monomer to hexamer around 1 μM pro...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2012
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| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3303956/ https://ncbi.nlm.nih.gov/pubmed/22326348 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jmb.2012.02.009 |
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