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Permanent or reversible conjugation of 2′-O- or 5′-O-aminooxymethylated nucleosides with functional groups as a convenient and efficient approach to the modification of RNA and DNA sequences

2′-O-Aminooxymethyl ribonucleosides are prepared from their 3′,5′-disilylated 2′-O-phthalimidooxymethyl derivatives by treatment with NH(4)F in MeOH. The reaction of these novel ribonucleosides with 1-pyrenecarboxaldehyde results in the efficient formation of stable and yet reversible ribonucleoside...

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Autors principals: Cieślak, Jacek, Grajkowski, Andrzej, Ausín, Cristina, Gapeev, Alexei, Beaucage, Serge L.
Format: Artigo
Idioma:Inglês
Publicat: Oxford University Press 2012
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC3300013/
https://ncbi.nlm.nih.gov/pubmed/22067450
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkr896
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