Polymerase chain reaction facilitates the cloning, CDR-grafting, and rapid expression of a murine monoclonal antibody directed against the CD18 component of leukocyte integrins.

Two novel approaches of recombinant PCR technology were employed to graft the complementarity determining regions from a murine monoclonal antibody (mAb) onto human antibody frameworks. One approach relied on the availability of cloned human variable region templates, whereas the other strategy was...

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Publié dans:	Nucleic Acids Res
Auteurs principaux:	Daugherty, B L, DeMartino, J A, Law, M F, Kawka, D W, Singer, I I, Mark, G E
Format:	Artigo
Langue:	Inglês
Publié:	Oxford University Press 1991
Accès en ligne:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC329459/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1674999/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/19.9.2471
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