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A Rapid FACS-Based Strategy to Isolate Human Gene Knockin and Knockout Clones

Gene targeting protocols for mammalian cells remain inefficient and labor intensive. Here we describe FASTarget, a rapid, fluorescent cell sorting based strategy to isolate rare gene targeting events in human somatic cells. A fluorescent protein is used as a means for direct selection of targeted cl...

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Detalles Bibliográficos
Autores principales: Mata, João F., Lopes, Telma, Gardner, Rui, Jansen, Lars E. T.
Formato: Artigo
Lenguaje:Inglês
Publicado: Public Library of Science 2012
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC3290580/
https://ncbi.nlm.nih.gov/pubmed/22393430
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0032646
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