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A Rapid FACS-Based Strategy to Isolate Human Gene Knockin and Knockout Clones
Gene targeting protocols for mammalian cells remain inefficient and labor intensive. Here we describe FASTarget, a rapid, fluorescent cell sorting based strategy to isolate rare gene targeting events in human somatic cells. A fluorescent protein is used as a means for direct selection of targeted cl...
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| Autori principali: | , , , |
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| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
Public Library of Science
2012
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3290580/ https://ncbi.nlm.nih.gov/pubmed/22393430 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0032646 |
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