FLIM-FRET Imaging of Caspase-3 Activity in Live Cells Using Pair of Red Fluorescent Proteins

We report a new technique to detect enzyme activity inside cells. The method based on Fluorescence Lifetime Imaging (FLIM) technology allows one to follow sensor cleavage by proteolytic enzyme caspase-3. Specifically, we use the FLIM FRET of living cells via the confocal fluorescence microscopy. A s...

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Bibliografiske detaljer
Main Authors: Savitsky, Alexander P., Rusanov, Alexander L., Zherdeva, Victoria V., Gorodnicheva, Tatiana V., Khrenova, Maria G., Nemukhin, Alexander V.
Format: Artigo
Sprog:Inglês
Udgivet: Ivyspring International Publisher 2012
Fag:
Research Paper
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3287422/
https://ncbi.nlm.nih.gov/pubmed/22375160
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.7150/thno.3885
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