Chemical ligation of DNA: the first non-enzymatic assembly of a biologically active gene.
An artificial gene comprising 183 base pairs has been assembled by template-directed condensation of 35- to 53-membered oligodeoxyribo nucleotides with cyanogen bromide as a condensing agent. The reaction is complete within several minutes at 0 degrees C in buffer. The resulting mini-gene was cloned...
Guardat en:
| Publicat a: | Nucleic Acids Res |
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| Autors principals: | , , , , , , , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
Oxford University Press
1991
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| Accés en línia: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC328569/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1870979/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/19.15.4247 |
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