Chemical ligation of DNA: the first non-enzymatic assembly of a biologically active gene.

An artificial gene comprising 183 base pairs has been assembled by template-directed condensation of 35- to 53-membered oligodeoxyribo nucleotides with cyanogen bromide as a condensing agent. The reaction is complete within several minutes at 0 degrees C in buffer. The resulting mini-gene was cloned...

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Detalhes bibliográficos
Main Authors: Shabarova, Z A, Merenkova, I N, Oretskaya, T S, Sokolova, N I, Skripkin, E A, Alexeyeva, E V, Balakin, A G, Bogdanov, A A
Formato: Artigo
Idioma:Inglês
Publicado em: 1991
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC328569/
https://ncbi.nlm.nih.gov/pubmed/1870979
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