Chemical ligation of DNA: the first non-enzymatic assembly of a biologically active gene.

An artificial gene comprising 183 base pairs has been assembled by template-directed condensation of 35- to 53-membered oligodeoxyribo nucleotides with cyanogen bromide as a condensing agent. The reaction is complete within several minutes at 0 degrees C in buffer. The resulting mini-gene was cloned...

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Detalles Bibliográficos

Publicado en:	Nucleic Acids Res
Principais autores:	Shabarova, Z A, Merenkova, I N, Oretskaya, T S, Sokolova, N I, Skripkin, E A, Alexeyeva, E V, Balakin, A G, Bogdanov, A A
Formato:	Artigo
Idioma:	Inglês
Publicado:	Oxford University Press 1991
Acceso en liña:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC328569/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1870979/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/19.15.4247
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