Optimization of oligonucleotide arrays and RNA amplification protocols for analysis of transcript structure and alternative splicing

Microarrays offer a high-resolution means for monitoring pre-mRNA splicing on a genomic scale. We have developed a novel, unbiased amplification protocol that permits labeling of entire transcripts. Also, hybridization conditions, probe characteristics, and analysis algorithms were optimized for det...

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Autors principals: Castle, John, Garrett-Engele, Phil, Armour, Christopher D, Duenwald, Sven J, Loerch, Patrick M, Meyer, Michael R, Schadt, Eric E, Stoughton, Roland, Parrish, Mark L, Shoemaker, Daniel D, Johnson, Jason M
Format: Artigo
Idioma:Inglês
Publicat: BioMed Central 2003
Matèries:
Method
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC328455/
https://ncbi.nlm.nih.gov/pubmed/14519201
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